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Objective To investigate the biological function of NS5ABP37 and to look for proteins interacting with NS5ABP37 protein in hepatocytes. Methods We constructed bait plasmid expressing NS5ABP37 protein of hepatitis C virus (HCV) by cloning the gene of NS5ABP37 protein into pGBKT7, then the recombinant plasmid DNA was transformed into yeast AH109 (α type). The transformed yeast AH109 was mated with yeast Y187 (α type) containing liver cDNA library plasmid in 2×YPDA medium. Diploid yeast was plated on synthetic dropout nutrient medium (SD/-Trp-Leu-His-Ade) containing X-α-gal for selection and screening. After extracting and sequencing of plasmids from positive (blue) colonies, we made a sequence analysis by bioinformatics. Results We screened twenty-five proteins binding to NS5ABP37, including Homo sapiens cyclin Ⅰ (CCNI) gene, Homo sapiens matrix metallopeptidase 25 (MMP25) and Homo sapiens talin 1. Conclusion The yeast-two hybrid system is an effective method for identifying hepatocyte proteins interacting with NS5ABP37 of HCV. And the biological function of NS5ABP37 may be associated with glycometabolism, lipid metabolism and apoptosis. 相似文献
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Fuel economy comparison of conventional drive trains series and parallel hybrid electric step vans 总被引:1,自引:0,他引:1
S. B. Han Y. H. Chang Y. J. Chung E. Y. Lee B. Suh A. Frank 《International Journal of Automotive Technology》2009,10(2):235-240
A hybrid electric vehicle (HEV) is a vehicle that combines a conventional propulsion system with an on-board rechargeable
energy storage system to achieve better fuel economy than a conventional vehicle HEVs do not have limited ranges like battery
electric vehicles, which use batteries charged by an external source. The different propulsion power systems may have common
subsystems or components. The objective of this study is to compare the fuel economies of a conventional step van, a series
hybrid electric step van (HESV), and a parallel HESV by calculating the fuel consumption using the ADVISOR software by NREL.
We also showed the results of the vehicles in different driving cycles including the Central Business District bus cycles,
the New York City Cycle, and the US EPA City and Highway cycles. 相似文献
45.
对16根混凝土梁进行抗裂试验研究和数值模拟,分析其开裂弯矩与纤维体积掺量的关系,并将试验结果与有限元分析结果进行对比.结果表明,适量加入钢-聚丙烯混杂纤维,可提高混凝土梁正截面开裂弯矩,并且随着钢纤维体积率的增加呈增长趋势,当聚丙烯纤维体积率固定为0.055%、0.11%、0.165%,钢纤维体积率达到最高1%时,其抗裂弯矩分别比普通高性能混凝土梁提高了26%、42.9%、26%,同时,影响其抗裂度大小的主要因素为钢纤维的体积掺量. 相似文献
46.
新建安九铁路鳊鱼洲长江大桥采用两线350 km/h高铁及两线200 km/h客货共线标准建设。南汊航道桥采用主跨672 m双塔钢箱混合梁交叉索斜拉桥,北汊航道桥采用主跨2×140 m独塔竖琴式预应力混凝土梁斜拉桥,鳊鱼洲及北汊非通航孔区采用48 m简支梁桥,跨北岸大堤采用主跨100 m变高连续梁桥,南、北岸引桥除连续梁外均采用32 m、24 m铁路标准梁,基础均采用桩基础。其中南、北汊航道桥采用整幅修建,其余采用分幅修建。桥址区基岩为灰岩,岩溶发育,设计采用了预注浆及抛填片石粘土法或灌注低标号片石混凝土措施进行处理。 相似文献
47.
统计能量法(SEA)在航空航天和船舶领域进行动力学环境预报方面有着广泛应用,适用于大型、复杂结构与高频率激励环境.但该方法在计算中低频振动噪声时,由于众多子系统动力特性差异,动力响应计算将产生很大误差,出现SEA失效问题.近年来出现的FE-SEA混合模型方法可有效解决这一问题,并能实现大型复杂结构的全频域一体化建模,在统一平台上进行低频、中频和高频计算及结果输出,简化建模和不同软件下模型转换,是非常适用于工程的一种方法.本研究探索采用具有上述功能的VA One软件进行舰艇基座全频域混和法动力学预报,这是国内外的首次探索,具有重要学术与工程价值. 相似文献
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《Vehicle System Dynamics: International Journal of Vehicle Mechanics and Mobility》2012,50(10):1518-1532
In this paper, a hybrid hydraulic passenger car (HHPC) coupled with a power split continuously variable transmission (P-CVT) is proposed. This P-CVT is capable of splitting the power from the internal combustion engine into mechanical and hydraulic power flows. By adjusting the ratio of the mechanical power to hydraulic power, the P-CVT enables the transmission ratio to be changed continuously. Meanwhile, the P-CVT system can capture the braking energy and store it in the hydraulic accumulator for the next assistant driving. In order to quantitatively investigate the effect of applying P-CVT on improving the fuel economy and operating performance for the HHPC, a numerical simulation is conducted under typical city driving conditions. The simulation results demonstrate that, the P-CVT permits the engine to be run under a more efficient operating range. The total fuel consumption of the HHPC is reduced by 16.4% under the test conditions, compared with that of the original car. 相似文献